Xylo-oligosaccharides (XOS) are a class of prebiotic fibers derived from the enzymatic hydrolysis of xylan, a hemicellulose present in plant cell walls. Comprising short chains of xylose molecules, XOS are resistant to digestion in the upper gastrointestinal tract and reach the colon intact, where they serve as a substrate for beneficial gut bacteria. Recognized for their prebiotic properties, XOS selectively stimulate the growth and activity of probiotics, particularly bifidobacteria. Found naturally in certain fruits, vegetables, and plant-based materials, XOS is increasingly utilized as an ingredient in functional foods and dietary supplements. Its prebiotic effects contribute to the maintenance of a balanced gut microbiota, supporting digestive health and potentially conferring additional health benefits. XOS is valued for its ability to enhance the fiber content of products while offering a mild sweetness, making it suitable for a variety of applications in the food and beverage industry.