Feed grade L-Isoleucine is a crucial amino acid supplement in animal nutrition, particularly in the feed industry. As an essential branched-chain amino acid (BCAA), L-Isoleucine plays a fundamental role in protein synthesis, supporting optimal growth, muscle development, and overall metabolic functions in livestock and poultry. Its significance arises from being indispensable for various physiological processes that contribute to the well-being of animals. Since animals cannot synthesize L-Isoleucine internally, its inclusion in their diets becomes essential. The supplementation of feed with L-Isoleucine is known to enhance the overall amino acid profile, promote protein utilization, and support the health and productivity of various animal species.